ABSTRACT
The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin (300 ng/kg chow) for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS. Treated rats showed extensive areas of chondrocyte necrosis and superficial cartilage defect in the articular cartilage. In addition, 8 metabolites were found to change significantly in these rats compared to the control group, including lysoPE (18:0/0:0), lysoPC(14:0), lysoPC[18:4 (6Z,9Z,12Z,15Z)], lysoPC[(16:1(9Z)], lysoPC(16:0), L-valine, hippuric acid, and asparaginyl-glycine. These 8 metabolites associated with cartilage injury are mainly involved in phospholipid and amino acid metabolic pathways.
ABSTRACT
Objective The aim of our study is to establish a proper performance evaluation index system of community disease control and prevention for both urban and rural areas in Hangzhou city. Methods The evaluation indexes were selected by Delphi method with two rounds,and the weights of the indexes were determined by analytical hierarchy process. Results A total of 3 first grade indexes were selected with indexes weight of 0. 18,0. 46 and 0. 36,11 second grade indexes were selected of which 4 indexes including funds guarantee,disease control and prevention,immunization program and work performance had relative high weight of 0. 46,0. 24,0. 29 and 0. 62 respectively. Moreover,50 third grade indexes were determined and the indexes considering traditional disease control and prevention had higher indexes weight. Conclusion The newly index system have reasonable structure and comprehensive contents. It could be used to guide the practical work of community disease control and prevention in Hangzhou city.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the pollution of hexavalent chromium in the electroplating workplace and screen the biomarkers of chromium exposure.</p><p><b>MATERIAL</b>Field occupational health investigation was conducted in 25 electroplating workplaces. 157 electroplating workers and 93 healthy unexposed controls were recruited. The epidemiological information was collected with face to face interview. Chromium in erythrocytes was determined by graphite furnace atomic absorption spectrophotometer.</p><p><b>RESULTS</b>The median of short-term exposure concentration of chromium in the air at electroplating workplace was 0.06 mg/m(3) (median) and ranging from 0.01 (detect limit) to 0.53 mg/m(3)). The median concentration of Cr (VI) in erythrocytes in electroplating workers was 4.41 (2.50 ∼ 5.29) µg/L, which was significantly higher than that in control subjects [1.54 (0.61 ∼ 2.98) µg/L, P < 0.01]. After stratified by potential confounding factors such as gender, age, smoking status and alcohol consumption, significant differences still existed between electroplating workers and control subjects, except for the subjects of age less than 30 years old (P = 0.11).</p><p><b>CONCLUSION</b>There was hexavalent chromium pollution in electroplating workplace. Occupational hazards prevention measures should be taken to control the chromium pollution hazards.</p>